|
KMID : 0364820210570030169
|
|
Korean Journal of Microbiology
2021 Volume.57 No. 3 p.169 ~ p.173
|
|
|
Enhancement of antibody-mediated botulinum toxin A neutralization by conjugating annexin V to the antibody in the mouse model
|
|
Choi Eun-Sun
Jeon Jun-Ho
Kim Yu-Ri
Kim Yeon-Hee
Cha Ki-Weon
Rhie Gi-Eun
|
|
|
Abstract
|
|
|
|
Botulism is a neuroparalytic illness caused by the exotoxin of Clostridium botulinum. Although equine antitoxin or human immunoglobulin has been used for the treatment of botulism, development of a new antibody therapeutics is required to minimize side effects. We designed an apoptotic cell-targeting protein complex to link Annexin V to Phosphatidylserine (PS) on the host’s apoptotic cells to enhance Botulinum Toxin (BoNT) neutralization. The apoptotic cell-targeting protein complex is composed of biotinylated H9-6 antibody (bH9-6) that specifically binds to heavy chain of BoNT/A and Streptavidin-Linker-fused Annexin V protein (StAv-L-Annexin V) that targets the PS of host’s apoptotic cells. In the mouse model, bH9-6-StAv-L-Annexin V complex completely protected mice against 5 LD50 of BoNT/A, whereas the bH9-6 antibody-treated group did not show any neutralizing ability. These results suggest that bH9-6-StAv-L-Annexin V complex might enhance BoNT/A neutralization by binding to apoptotic cells. Further study is required to clarify the exact clearance mechanism.
|
|
|
KEYWORD
|
|
|
antibody, annexin V, botulism, botulinum toxin A, neutralization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|